Construction and characterization of human embryonic kidney-(HEK)-293T cell overexpressing truncated α4 integrin
نویسندگان
چکیده
منابع مشابه
Construction and Characterization of Recombinant HEK Cell Over Expressing α4 Integrin.
PURPOSE Integrins are heterodimeric membrane proteins, which are exposed to post translational modifications in eukaryotic cells in contrast to prokaryotic cells. These modifications provide advantages for production of proper nanobody, mono and polyclonal antibody against this surface protein and also in aptamer selection process. Since the majority of diagnostic and therapeutic antibodies, ta...
متن کاملCloning, Expression and Characterization of Zebra Fish Ferroportin in Hek 293T Cell Line
BACKGROUND Ferroportin (Fpn), a regulator of iron homeostasis is a conserved membrane protein that exports iron across the enterocytes, macrophages and hepatocytes into the blood circulation. Fpn has also critical influence on survival of microorganisms whose growth is dependent upon iron, thus preparation of Fpn is needed to study the role of iron in immunity and pathogenesis of micoorganisms....
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Disruption of Golgi alpha-mannosidase II activity can result in type II congenital dyserythropoietic anemia and induce lupus-like autoimmunity in mice. Here, we isolated a mutant human embryonic kidney (HEK) 293T cell line called Lec36, which displays sensitivity to ricin that lies between the parental HEK 293T cells, in which the secreted and membrane-expressed proteins are dominated by comple...
متن کاملHuman Elongator complex is involved in cell cycle and suppresses cell growth in 293T human embryonic kidney cells.
Elongator complex has been associated with hyperphosphorylated RNA polymerase II and is known to play critical roles in transcriptional elongation, as well as in tRNA modification and exocytosis. However, the specific mechanism of how human Elongator complex regulates cell growth and cell cycle remains unclear. To investigate the composition of human Elongator complex and its effects on cell gr...
متن کاملcloning, expression and characterization of zebra fish ferropor¬tin in hek 293t cell line
background: ferroportin (fpn), a regulator of iron homeostasis is a conserved membrane protein that exports iron across the enterocytes, macrophages and hepatocytes into the blood circulation. fpn has also critical influence on survival of microorganisms whose growth is dependent upon iron, thus preparation of fpn is needed to study the role of iron in immunity and pathogenesis of micoorganisms...
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ژورنال
عنوان ژورنال: Research in Pharmaceutical Sciences
سال: 2018
ISSN: 1735-5362
DOI: 10.4103/1735-5362.235162